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Western blot(SDS PAGE) analysis of extracts from HL60 cells .Using p40-phoxRabbit mAb [C446] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NCF4,SH3PXD4,NCF4_HUMAN,Neutrophil cytosol factor 4
UniProtID :Q15080
MASS(da) :39,032
MW(kDa) :39 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human p40-phox
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HL60 cells
Function : Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex..
Tissue specificity :Expression is restricted to hematopoietic cells.
Subcellular locationi :Cytoplasm, cytosol. Endosome membrane,Peripheral membrane protein,Cytoplasmic side. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.