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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells .Using PEX14 Rabbit mAb [EVFP] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PEX14,PEX14_HUMAN,Peroxisomal membrane protein PEX14
UniProtID :O75381
MASS(da) :41,237
MW(kDa) :57 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PEX14.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 63% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Peroxisome membrane protein that is an essential component of the peroxisomal import machinery. Functions as a docking factor for the predominantly cytoplasmic PTS1 receptor (PEX5). Plays a key role for peroxisome movement through a direct interaction with tubulin..
Subcellular locationi :Peroxisome membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.