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Western blot(SDS PAGE) analysis of extracts from GM-CSF treated TF-1 .Using P-STAT5A (Tyr694) Rabbit mAb [C2S3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :STAT5A,STAT5,STA5A_HUMAN,Signal transducer and activator of transcription 5A
UniProtID :P42229
MASS(da) :90,647
MW(kDa) :95
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human P-STAT5A (Tyr694).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 70% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :GM-CSF treated TF-1
Function : Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.