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Western blot(SDS PAGE) analysis of extracts from THP-1 cells .Using BCL2 Rabbit mAb [414M] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCL2,BCL2_HUMAN,Apoptosis regulator Bcl-2
UniProtID :P10415
MASS(da) :26,266
MW(kDa) :26
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human BCL2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 71% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cells
Function : Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785)..
Tissue specificity :Expressed in a variety of tissues.
Subcellular locationi :Mitochondrion outer membrane,Single-pass membrane protein. Nucleus membrane,Single-pass membrane protein. Endoplasmic reticulum membrane,Single-pass membrane protein. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.