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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TOM20 Rabbit mAb [62DH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TOMM20,KIAA0016,TOM20_HUMAN,Mitochondrial import receptor subunit TOM20 homolog
UniProtID :Q15388
MASS(da) :16,298
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TOM20.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 94% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases..
Subcellular locationi :Mitochondrion outer membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.