* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HEK-293 cells .Using Occludin Rabbit mAb [WZZZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :OCLN,OCLN_HUMAN,Occludin
UniProtID :Q16625
MASS(da) :59,144
MW(kDa) :59
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Occludin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 98% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.., (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes..
Tissue specificity :Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell junction, tight junction.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.