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Western blot(SDS PAGE) analysis of extracts from mouse brain .Using SYNPO Rabbit mAb [RDO5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SYNPO,KIAA1029,SYNPO_HUMAN,Synaptopodin
UniProtID :Q8N3V7
MASS(da) :99,463
MW(kDa) :100
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SYNPO.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 99% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse brain
Function : Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity)..
Tissue specificity :Expressed in cerebral cortex..
Subcellular locationi :Cytoplasm, cytoskeleton. Cell junction, tight junction. Perikaryon. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.