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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using CD45 Rabbit mAb [TOSY] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PTPRC,CD45,PTPRC_HUMAN,Receptor-type tyrosine-protein phosphatase C
UniProtID :P08575
MASS(da) :147,486
MW(kDa) :220
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD45.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 100% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).., (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation..
Tissue specificity :Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Membrane raft.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.