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Western blot(SDS PAGE) analysis of extracts from HEK293 treated with Calyculin A .Using P-RPS6 (S236) Rabbit mAb [7P8U] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RPS6,OK/SW-cl.2,RS6_HUMAN,40S ribosomal protein S6
UniProtID :P62753
MASS(da) :28,681
MW(kDa) :32
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human P-RPS6 (S236).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 102% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 treated with Calyculin A
Function : Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.