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Western blot(SDS PAGE) analysis of extracts from mouse brain.Using Beta Tubulin Rabbit mAb [1O91] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TUBB3,TUBB4,TBB3_HUMAN,Tubulin beta-3 chain
UniProtID :Q13509
MASS(da) :50,433
MW(kDa) :55
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Beta Tubulin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 106% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse brain
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977)..
Tissue specificity :Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues..
Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.