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Western blot(SDS PAGE) analysis of extracts from Rat spleen.Using active + pro Caspase-3 Rabbit mAb [HHET] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CASP3,CPP32,CASP3_HUMAN,Caspase-3 [Cleaved into: Caspase-3 subunit p17,Caspase-3 subunit p12]
UniProtID :P42574
MASS(da) :31,608
MW(kDa) : 32KDa、17KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human active + pro Caspase-3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 109% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat spleen
Function : Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a
Tissue specificity :Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.