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active + pro Caspase-3 Rabbit mAb [HHET]Cat NO.: A71383

Western blot(SDS PAGE) analysis of extracts from Rat spleen.Using active + pro Caspase-3 Rabbit mAb [HHET] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CASP3,CPP32,CASP3_HUMAN,Caspase-3 [Cleaved into: Caspase-3 subunit p17,Caspase-3 subunit p12]

UniProtID :P42574

MASS(da) :31,608

MW(kDa) : 32KDa、17KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human active + pro Caspase-3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 109% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Rat spleen

Function : Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a

Tissue specificity :Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.