* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from MCF7 cells.Using CDKN1A/p21CIP1 Rabbit mAb [Z72S] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDKN1A,CAP20,CDKN1,CIP1,MDA6,PIC1,SDI1,WAF1,CDN1A_HUMAN,Cyclin-dependent kinase inhibitor 1
UniProtID :P38936
MASS(da) :18,119
MW(kDa) : 21kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CDKN1A/p21CIP1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 118% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 cells
Function : May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657)..
Tissue specificity :Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.