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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Cyclin B1 Rabbit mAb [S87Y] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CCNB1,CCNB,CCNB1_HUMAN,G2/mitotic-specific cyclin-B1
UniProtID :P14635
MASS(da) :48,337
MW(kDa) :55kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cyclin B1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 121% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Essential for the control of the cell cycle at the G2/M (mitosis) transition..
Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.