Fas Rabbit mAb [WY3S]Cat NO.: A47758

Protein names ：FAS,APT1,FAS1,TNFRSF6,TNR6_HUMAN,Tumor necrosis factor receptor superfamily member 6

UniProtID ：P25445

MASS(da) ：37,732

MW(kDa) ：48

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000,
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Fas.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 127% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro)..

Tissue specificity ：Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6..

Subcellular locationi ：[Isoform 1]: Cell membrane,Single-pass type I membrane protein. Membrane raft.,[Isoform 2]: Secreted.,[Isoform 3]: Secreted.,[Isoform 4]: Secreted.,[Isoform 5]: Secreted.,[Isoform 6]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.