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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GATA2 Rabbit mAb [RDZN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GATA2,GATA2_HUMAN,Endothelial transcription factor GATA-2
UniProtID :P23769/P23771
MASS(da) :50,500
MW(kDa) :51
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GATA2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 130% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity :Endothelial cells.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.