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Hexokinase II Rabbit mAb [RHOQ]Cat NO.: A14955

Western blot(SDS PAGE) analysis of extracts from Mouse heart.Using Hexokinase II Rabbit mAb [RHOQ] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :HK2,HXK2_HUMAN,Hexokinase-2

UniProtID :P52789

MASS(da) :102,380

MW(kDa) :102

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Hexokinase II.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 137% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse heart

Function : Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175)..

Tissue specificity :Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle..

Subcellular locationi :Mitochondrion outer membrane,Peripheral membrane protein. Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.