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Western blot(SDS PAGE) analysis of extracts from Mouse heart.Using Hexokinase II Rabbit mAb [RHOQ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HK2,HXK2_HUMAN,Hexokinase-2
UniProtID :P52789
MASS(da) :102,380
MW(kDa) :102
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Hexokinase II.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 137% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse heart
Function : Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175)..
Tissue specificity :Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle..
Subcellular locationi :Mitochondrion outer membrane,Peripheral membrane protein. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.