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Western blot(SDS PAGE) analysis of extracts from C6 cells were treated with Chloroquine.Using LC3B Rabbit mAb [7T2L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAP1LC3B,MAP1ALC3,MLP3B_HUMAN,Microtubule-associated proteins 1A/1B light chain 3B
UniProtID :Q9GZQ8
MASS(da) :14,688
MW(kDa) :14 16
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human LC3B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 144% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C6 cells were treated with Chloroquine
Function : Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355)..
Tissue specificity :Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver..
Subcellular locationi :Cytoplasmic vesicle, autophagosome membrane,Lipid-anchor. Endomembrane system,Lipid-anchor. Mitochondrion membrane,Lipid-anchor. Cytoplasm, cytoskeleton. Cytoplasmic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.