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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MEK2 Rabbit mAb [G8C4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAP2K2,MEK2,MKK2,PRKMK2,MP2K2_HUMAN,Dual specificity mitogen-activated protein kinase kinase 2
UniProtID :P36507
MASS(da) :44,424
MW(kDa) :44
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MEK2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 145% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner,by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126)..
Subcellular locationi :Cytoplasm. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.