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Smad1 Rabbit mAb [M77K]Cat NO.: A76625

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Smad1 Rabbit mAb [M77K] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SMAD1,BSP1,MADH1,MADR1,SMAD1_HUMAN,Mothers against decapentaplegic homolog 1

UniProtID :Q15797

MASS(da) :52,260

MW(kDa) :58

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Smad1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 156% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression..

Tissue specificity :Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.