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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Smad1 Rabbit mAb [M77K] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SMAD1,BSP1,MADH1,MADR1,SMAD1_HUMAN,Mothers against decapentaplegic homolog 1
UniProtID :Q15797
MASS(da) :52,260
MW(kDa) :58
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Smad1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 156% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression..
Tissue specificity :Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.