* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Smad2 Rabbit mAb [W332] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SMAD2,MADH2,MADR2,SMAD2_HUMAN,Mothers against decapentaplegic homolog 2
UniProtID :Q15796
MASS(da) :52,306
MW(kDa) :58
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Smad2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 157% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator..
Tissue specificity :Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.