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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using STAT5B Rabbit mAb [OC0Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :STAT5B,STA5B_HUMAN,Signal transducer and activator of transcription 5B
UniProtID :P51692
MASS(da) :89,866
MW(kDa) :90
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human STAT5B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 164% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Carries out a dual signal transduction and activation of transcription (PubMed:29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.