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α-Catenin Rabbit mAb [8JGE]Cat NO.: A83180

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using α-Catenin Rabbit mAb [8JGE] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CTNNA1,CTNA1_HUMAN,Catenin alpha-1

UniProtID :P35221

MASS(da) :100,071

MW(kDa) :100

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human α-Catenin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 168% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation..

Tissue specificity :Expressed ubiquitously in normal tissues.

Subcellular locationi :[Isoform 1]: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cell junction.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.