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Western blot(SDS PAGE) analysis of extracts from Mouse brain lysates.Using Tyrosine Hydroxylase Rabbit mAb IgG [9G11] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TH,TYH,TY3H_HUMAN,Tyrosine 3-monooxygenase
UniProtID :P07101
MASS(da) :58,600
MW(kDa) :59
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Tyrosine Hydroxylase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain lysates
Function : Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:17391063, PubMed:1680128, PubMed:15287903, PubMed:8528210, Ref.18, PubMed:34922205, PubMed:24753243). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).., [Isoform 5]: Lacks catalytic activity.., [Isoform 6]: Lacks catalytic activity..
Tissue specificity :Mainly expressed in the brain and adrenal glands.
Subcellular locationi :Cytoplasm, perinuclear region. Nucleus. Cell projection, axon. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.