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Western blot(SDS PAGE) analysis of extracts from mouse prostate lysates.Using ORP150 Rabbit mAb IgG [0V3J] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HYOU1,GRP170,ORP150,HYOU1_HUMAN,Hypoxia up-regulated protein 1
UniProtID :Q9Y4L1
MASS(da) :111,335
MW(kDa) :120
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ORP150.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse prostate lysates
Function : Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding..
Tissue specificity :Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.