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MyoD1 Rabbit mAb[CH38]Cat NO.: A73718

Western blot(SDS PAGE) analysis of extracts from Hela cells lysates.Using MyoD1 Rabbit mAb IgG [CH38] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MYOD1,BHLHC1,MYF3,MYOD,MYOD1_HUMAN,Myoblast determination protein 1

UniProtID :P15172

MASS(da) :34,501

MW(kDa) :45

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MyoD1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Hela cells lysates

Function : Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.