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Western blot(SDS PAGE) analysis of extracts from Human liver tissue lysate lysates.Using Junctional Adhesion Molecule 1 Rabbit mAb IgG [0RG5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :F11R,JAM1,JCAM,UNQ264/PRO301,JAM1_HUMAN,Junctional adhesion molecule A
UniProtID :Q9Y624
MASS(da) :32,583
MW(kDa) :33
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Junctional Adhesion Molecule 1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human liver tissue lysate lysates
Function : Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed:11812992). Involved in platelet activation (PubMed:10753840).., (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.., (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa..
Tissue specificity :Expressed in endothelium, epithelium and leukocytes (at protein level)..
Subcellular locationi :Cell junction, tight junction. Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.