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Western blot(SDS PAGE) analysis of extracts from A549 cells lysates.Using Sodium Potassium ATPase Rabbit mAb IgG [GJ8D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ATP1A1,AT1A1_HUMAN,Sodium/potassium-transporting ATPase subunit alpha-1 /K ATPase alpha-1 subunit)
UniProtID :P05023
MASS(da) :112,896
MW(kDa) :100
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Sodium Potassium ATPase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells lysates
Function : This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients..
Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Cell membrane, sarcolemma,Multi-pass membrane protein. Cell projection, axon. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.