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Lactate Dehydrogenase Rabbit mAb[2DS6]Cat NO.: A42494

Western blot(SDS PAGE) analysis of extracts fromMCF-7 cell lysate lysates.Using Lactate Dehydrogenase Rabbit mAb IgG [2DS6] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LDHB,LDHB_HUMAN,L-lactate dehydrogenase B chain

UniProtID :P07195

MASS(da) :36,638

MW(kDa) :37

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Lactate Dehydrogenase.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : MCF-7 cell lysate lysates

Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..

Tissue specificity :Predominantly expressed in aerobic tissues such as cardiac muscle..

Subcellular locationi :Cytoplasm. Mitochondrion inner membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.