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Western blot(SDS PAGE) analysis of extracts from human skin lysates.Using Mast Cell Tryptase Rabbit mAb IgG [0X18] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TPSAB1,TPS1,TPS2,TPSB1,TRYB1_HUMAN,Tryptase alpha/beta-1
UniProtID :Q15661
MASS(da) :30,515
MW(kDa) :30
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mast Cell Tryptase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human skin lysates
Function : Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315)..
Tissue specificity :Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin,in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level)..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.