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Western blot(SDS PAGE) analysis of extracts from Hela cells lysates.Using Calnexin Rabbit mAb IgG [30GD] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CANX,CALX_HUMAN,Calnexin
UniProtID :P27824
MASS(da) :67,568
MW(kDa) :90
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Calnexin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hela cells lysates
Function : Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.