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Western blot(SDS PAGE) analysis of extracts from mouse skeletal muscle lysates.Using Dysferlin Rabbit mAb IgG [1SEG] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DYSF,FER1L1,DYSF_HUMAN,Dysferlin
UniProtID :O75923
MASS(da) :237,295
MW(kDa) :237
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Dysferlin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse skeletal muscle lysates
Function : Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity)..
Tissue specificity :Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Ubiquitous. Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas..
Subcellular locationi :Cell membrane, sarcolemma,Single-pass type II membrane protein. Cytoplasmic vesicle membrane,Single-pass type II membrane protein. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.