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Western blot(SDS PAGE) analysis of extracts from PC-12 cells lysates.Using Synaptophysin Rabbit mAb IgG [6K1E] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SYP,SYPH_HUMAN,Synaptophysin
UniProtID :P08247
MASS(da) :33,845
MW(kDa) :38
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Synaptophysin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-12 cells lysates
Function : Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity)..
Tissue specificity :Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3 (PubMed:8838578). Expressed in the putamen (at protein level) (PubMed:17296554)..
Subcellular locationi :Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Multi-pass membrane protein. Cell junction, synapse, synaptosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.