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Western blot(SDS PAGE) analysis of extracts from Hela cell lysate lysates.Using CDC7 Kinase Rabbit mAb IgG [59SR] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC7,CDC7L1,CDC7_HUMAN,Cell division cycle 7-related protein kinase
UniProtID :O00311
MASS(da) :63,888
MW(kDa) :65
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CDC7 Kinase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hela cell lysate lysates
Function : Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylate MCM2 and MCM3..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.