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Western blot(SDS PAGE) analysis of extracts from mouse heart tissue lysate lysates.Using alpha smooth muscle Actin Rabbit mAb IgG [P7IC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACTA2,ACTSA,ACTVS,GIG46,ACTA_HUMAN,Actin, aortic smooth muscle [Cleaved into: Actin, aortic smooth muscle, intermediate form]
UniProtID :P62736
MASS(da) :42,009
MW(kDa) :43
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha smooth muscle Actin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse heart tissue lysate lysates
Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.