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SMC3 Rabbit mAb[23R2]Cat NO.: A74167

Western blot(SDS PAGE) analysis of extracts from PC12 cells lysates.Using SMC3 Rabbit mAb IgG [23R2] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SMC3,BAM,BMH,CSPG6,SMC3L1,SMC3_HUMAN,Structural maintenance of chromosomes protein 3

UniProtID :Q9UQE7

MASS(da) :141,542

MW(kDa) :141

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SMC3.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :PC12 cells lysates

Function : Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement..

Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.