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Western blot(SDS PAGE) analysis of extracts from human lung lysates.Using CD32 Rabbit mAb IgG [3VH8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FCGR2A,CD32,FCG2,FCGR2A1,IGFR2,FCG2A_HUMAN,Low affinity immunoglobulin gamma Fc region receptor II-a
UniProtID :P12318
MASS(da) :35,001
MW(kDa) :45
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD32.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : human lung lysates
Function : Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens..
Tissue specificity :Found on monocytes, neutrophils and eosinophil platelets.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.