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Western blot(SDS PAGE) analysis of extracts from PC-12 cell lysate lysates.Using alpha Internexin Rabbit mAb IgG [ZZZX] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :INA,NEF5,AINX_HUMAN,Alpha-internexin
UniProtID :Q16352
MASS(da) :55,391
MW(kDa) :55
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha Internexin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-12 cell lysate lysates
Function : Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity)..
Tissue specificity :Found predominantly in adult CNS.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.