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P-Elk-1 (S383) Mouse mAb [F0N3]Cat NO.: A64202

Western blot(SDS PAGE) analysis of extracts from Elk-1 fusion protein expressed from E. coli without phosphorylation with purified Erk2 enzyme.Using P-Elk-1 (S383 mouse mAb[F0N3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ELK1,ELK1_HUMAN,ETS domain-containing protein Elk-1

UniProtID :P19419

MASS(da) :44,888

MW(kDa) :47 kDa

Form :Liquid

Purification :Protein A purification

Host :mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-Elk-1 (Ser383)

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Elk-1 fusion protein expressed from E. coli without phosphorylation with purified Erk2 enzyme

Function : Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity)..

Tissue specificity :Lung and testis.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.