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Western blot(SDS PAGE) analysis of extracts fromNIH/3T3 cell lysate lysates.Using CREB Rabbit mAb IgG [23ME] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CREB1,CREB1_HUMAN,Cyclic AMP-responsive element-binding protein 1
UniProtID :P16220
MASS(da) :35,136
MW(kDa) :40
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CREB.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : NIH/3T3 cell lysate lysates
Function : Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.