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AKAP14 Rabbit mAb[W2S9]Cat NO.: A25746

Western blot(SDS PAGE) analysis of extracts from A549 cells lysates.Using AKAP14 Rabbit mAb IgG [W2S9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :AKAP14,AKAP28,AKA28_HUMAN,A-kinase anchor protein 14

UniProtID :Q86UN6

MASS(da) :22,815

MW(kDa) :23

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human AKAP14.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells lysates

Function : Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Tissue specificity :Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.