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Western blot(SDS PAGE) analysis of extracts from A549 cells lysates.Using AKAP14 Rabbit mAb IgG [W2S9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AKAP14,AKAP28,AKA28_HUMAN,A-kinase anchor protein 14
UniProtID :Q86UN6
MASS(da) :22,815
MW(kDa) :23
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human AKAP14.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells lysates
Function : Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Tissue specificity :Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.