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Western blot(SDS PAGE) analysis of extracts from human lung tissue lysate lysates.Using Caspase-3 Rabbit mAb IgG [NU04] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CASP3,CPP32,CASP3_HUMAN,Caspase-3 [Cleaved into: Caspase-3 subunit p17,Caspase-3 subunit p12]
UniProtID :P42574
MASS(da) :31,608
MW(kDa) :32
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Caspase-3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human lung tissue lysate lysates
Function : Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430). At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a
Tissue specificity :Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.