* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from A549 cells lysates.Using ATP citrate lyase Rabbit mAb IgG [60GO] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACLY,ACLY_HUMAN,ATP-citrate synthase -lyase)
UniProtID :P53396
MASS(da) :120,839
MW(kDa) :125
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ATP citrate lyase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells lysates
Function : Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.