support:13376277882 | tech@naturebios.com

PDH E1 Alpha Mouse mAb[CIRO]Cat NO.: A71453

Western blot(SDS PAGE) analysis of extracts from HEK-293 cells lysates.Using PDH E1 Alpha mouse mAb IgG [CIRO] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
Order Datasheet

Product information

Protein names :PDHA1,PHE1A,ODPA_HUMAN,Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

UniProtID :P08559

MASS(da) :43,296

MW(kDa) :43

Form :Liquid

Purification :Protein A purification

Host :mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PDH E1 Alpha.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK-293 cells lysates

Function : The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle..

Tissue specificity :Ubiquitous.

Subcellular locationi :Mitochondrion matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.