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Western blot(SDS PAGE) analysis of extracts from 293T cells,transfected with human Atg13 and mouse ULK1 , ..Using Phospho-Atg13 (Ser355)Rabbit mAb IgG [287S] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ATG13,KIAA0652,ATG13_HUMAN,Autophagy-related protein 13
UniProtID :O75143
MASS(da) :56,572
MW(kDa) : 72
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser355 of human Atg13
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells,transfected with human Atg13 and mouse ULK1 , .
Function : Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation..
Subcellular locationi :Cytoplasm, cytosol. Preautophagosomal structure.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.