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Western blot(SDS PAGE) analysis of extracts from HeLa cells, serum-starved overnight, and treated with TPA ..Using Phospho-FRA1 (Ser265)Rabbit mAb IgG [9347] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FOSL1,FRA1,FOSL1_HUMAN,Fos-related antigen 1
UniProtID :P15407
MASS(da) :29,413
MW(kDa) : 40
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser265 of human FRA1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells, serum-starved overnight, and treated with TPA .
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.