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Western blot(SDS PAGE) analysis of extracts from jurkat cell lines, treated with hIFN-α1(10 ng/ml, 15 min) ..Using Phospho-Jak1(Tyr1034/1035)Rabbit mAb IgG [4892] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :JAK1,JAK1A,JAK1B,JAK1_HUMAN,Tyrosine-protein kinase JAK1
UniProtID :P23458
MASS(da) :133,277
MW(kDa) : 130
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1034 and Tyr1035 of human Jak1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :jurkat cell lines, treated with hIFN-α1(10 ng/ml, 15 min) .
Function : Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558, PubMed:28111307, PubMed:32750333, PubMed:16239216). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952). Directly phosphorylates STAT but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors (PubMed:16239216, PubMed:32750333)..
Tissue specificity :Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue..
Subcellular locationi :Endomembrane system,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.