Phospho-MKK3 (Ser189)/MKK6 (Ser207) Rabbit mAb[1XV5]Cat NO.: A51614

Protein names ：MAP2K3,MEK3,MKK3,PRKMK3,SKK2,MP2K3_HUMAN,Dual specificity mitogen-activated protein kinase kinase 3

UniProtID ：P46734 , P52564

MASS(da) ：39,318

MW(kDa) ： 38 MKK6, 40 MKK3

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser189 of human MKK3 /MKK6

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：NIH/3T3 cells,treated with TPA(200 nM, 15 min).

Function ： Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14..

Tissue specificity ：Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.