Phospho-Myosin Light Chain 2 (Thr18/Ser19) Rabbit mAb[L16S]Cat NO.: A63807

Protein names ：MYL9,MLC2,MRLC1,MYRL2,MYL9_HUMAN,Myosin regulatory light polypeptide 9

UniProtID ：P24844

MASS(da) ：19,827

MW(kDa) ： 18

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr18 and Ser19 of human Myosin Light Chain 2

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293 and HeLa cells.

Function ： Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity)..

Tissue specificity ：Smooth muscle tissues and in some, but not all, nonmuscle cells..

Subcellular locationi ：Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.