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Phospho-Paxillin (Tyr118) Rabbit mAb[0O69]Cat NO.: A99159

Western blot(SDS PAGE) analysis of extracts from HeLa cells in suspension,induced attachment to fibronectin-coated plate surface (10 μg/ml, 1 hr)..Using Phospho-Paxillin (Tyr118)Rabbit mAb IgG [0O69] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PXN,PAXI_HUMAN,Paxillin

UniProtID :P49023

MASS(da) :64,505

MW(kDa) : 68

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr118 of human Paxillin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells in suspension,induced attachment to fibronectin-coated plate surface (10 μg/ml, 1 hr).

Function : Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Subcellular locationi :Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.