Phospho-Tyk2 (Tyr1054/1055) Rabbit mAb[CP12]Cat NO.: A44709

Protein names ：TYK2,TYK2_HUMAN,Non-receptor tyrosine-protein kinase TYK2

UniProtID ：P29597

MASS(da) ：133,650

MW(kDa) ： 134

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1054 and Tyr1055 of human Tyk2

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：serum-starved U266 cells, treated with hIFN-α1 (50 ng/ml, 15 min) .

Function ： Non-receptor kinase involved in various processes including cell growth, development, cell migration, innate and adaptive immunity (PubMed:10995743, PubMed:10542297). Plays both structural and catalytic roles in numerous cytokines and interferons signaling (PubMed:10542297). Associates with cytokine and growth factor receptors and activate STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed:10542297, PubMed:7638186). The heterodimeric cytokine receptor complexes are composed of a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1) which serves as the signal transducing chain harboring STAT docking sites once phosphorylated by TYK2, and a second receptor chain associated either with JAK1 or JAK2 (PubMed:10542297, PubMed:7526154, PubMed:25762719). In turn, recruited STATs are phosphorylated, form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes. Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed:29162862)..

Tissue specificity ：Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.